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Friday, July 17, 2020 | History

2 edition of immunological method for the assay of chorionic gonadotrophin and its application. found in the catalog.

immunological method for the assay of chorionic gonadotrophin and its application.

Aileen F. Connon

immunological method for the assay of chorionic gonadotrophin and its application.

by Aileen F. Connon

  • 251 Want to read
  • 9 Currently reading

Published .
Written in English


Edition Notes

Thesis (M.D.)--The Queen"s University of Belfast, 1963.

The Physical Object
Pagination1 v
ID Numbers
Open LibraryOL19421341M

  International reference preparation of human chorionic gonadotrophin for immunoassay: Potency estimates in various bioassay and protein binding assay systems; and international reference preparations of α and β subunits of human chorionic gonadotrophin for immunoassay. J Endocrinol ; – Google Scholar | Medline | ISICited by: Structure of human chorionic gonadotropin at A resolution from MAD analysis of the selenomethionyl protein Hao Wu1, Joyce W Lustbader2, Yee Liu1, Robert E Canfield2 and Wayne A Hendricksonl,3* 'Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY , USA, 2Department of Medicine, Columbia University, New York, NY , USA.

Abstract. The last 10 years have seen a revolution in our understanding of the structure and functions of chorionic gonadotrophin (CG). Until the early seventies it was thought to be a glycoprotein hormone restricted to pregnancy in by: 7.   The assay is capable of reliably and accurately measuring as little as ng hCG/ml urine without interference from hLH. Assays of urine from normal men and nonpregnant women of reproductive age indicated that most individuals did not have detectable levels of hCG immunoreactivity, although a minority had minute amounts, with a mean value of Cited by:

The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science /5(7). amino acid sequence. Chorionic gonadotropin is obtained from the human pregnancy urine. It is standardized by a biological assay procedure. Chorionic Gonadotropin for Injection, USP is available in multiple dose vials contain USP Units with accompanying Bacteriostatic Water for File Size: 42KB.


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Immunological method for the assay of chorionic gonadotrophin and its application by Aileen F. Connon Download PDF EPUB FB2

Immunological assay 1. Presented by- Asha M. Jagtap. Rajarambapu college of pharmacy, kasegaon. cy (pharmaceutics) 2. An immunoassay is a biochemical test that measures the presence or concentration of a macromolecule or a small molecule in a solution through the use of an antibody (usually) or an antigen (sometimes).

So, to test this hypothesis an immunological method-namely a haemagglutination- inhibition test-was used.5 6 The specificity of the assay method for H.c.G. was demonstrated by the absence of inhibition of agglutination when non-pregnant urinary protein and human menopausal gonadotrophin extracts were Cited by: 8.

A highly sensitive assay for alkaline phosphatase (ALP) was developed using phenacyl phosphate as a substrate for chemiluminescent assays. This substrate is applied to two types of immunological assays based on ALP labelling, an assay for human chorionic gonadotrophin (hCG) in urine by a two-site immunometric assay and an assay for human prostatic acid phosphatase (PAP) adsorbed on a solid Cited by: Bioassay for anti-chorionic gonadotropin sera Article (PDF Available) in J Reprod Fertil 67(2) April with Reads How we measure 'reads'.

immunological assays - ELISA and RIA 1. IMMUNOLOGICAL ASSAYS FIRST (QUALITY ASSURANCE) SRINIVAS COLLEGE OF PHARMACY 1 2. IMMUNOASSAY • Immunoassay means a method to measure any particular substance in a mixture using its specific-binding antibody.

Akush Ginekol (Mosk). Dec;42(12) [The immunological method of quantitative determination of choriomic gonadotrophin in the urine].Author: Kotliarskaia Ei. Immunology and Serology i Preface Immunology and serology is an advanced science dealing with how the human immune system organized, function and the different types of serological techniques.

It is a very vast subject covering a wide area of technology. The. Immunoprecipitation: variations of the method.

Magnetic beads coupled to antibodies can be used to isolate proteins from solution, or cells from a suspension. And check out “A Toll-like receptor recognizes bacterial DNA” Hemmi Nature v p Figure 3 f and g For real-life examples of Immunoprecipitations and.

J Endocrinol. Apr;43(4) A quantitative immunological assay for pregnant mare serum gonadotrophin. Allen WR. PMID: [PubMed - indexed for MEDLINE]Cited by: Wide, L. An immunological method on the assay of human chorionic gonadotrophin.

Pregnancy-specific antigens in the sheep: application to the diagnosis of pregnancy. Reprod. 17I3, oestrone, testosterone, androstenedione, and chorionic gonadotrophin during pregnancy in the marmoset monkey, Callithrix jacchus.

Author: J. Findlay. An immunoassay is a biochemical test that measures the presence or concentration of a macromolecule or a small molecule in a solution through the use of an antibody (usually) or an antigen (sometimes).

The molecule detected by the immunoassay is often referred to as an "analyte" and is in many cases a protein, although it may be other kinds of molecules, of different size and types, as long as MeSH: D Human chorionic gonadotrophin (hCG) is the first specific molecule synthesized by the embryo.

hCG RNA is transcribed as early as the eight-cell stage, and the blastocyst produces the protein. gonadotropin [go´nah-do-tro″pin] any hormone having a stimulating effect on the gonads.

Two such hormones are secreted by the anterior pituitary gland: follicle-stimulating hormone and luteinizing hormone, both of which are active, but with differing effects, in the two sexes.

Called also gonadotropic hormone. chorionic gonadotropin (human chorionic. analytical method of high throughput (). The analy-sis of complex biological matrices (e.g. blood or urine) by immunoassay methods, being based on a specific bind-ing reaction, can be achieved without pretreatment for the sample ().Although the developing of a new immu-noassay method for an analyte may take months (due to theCited by:   The heterogeneity of human chorionic gonadotropin (hCG).

III. The occurrence and biological and immunological activities of nicked hCG. Endocrinology ; () Bock JL. HCG assays.

A plea for uniformity. Am J Clin Pathol ; () College of American Pathologists. Ligand assay series, Excel survey, CAP and » Chorionic Gonadotropin is a gonad-stimulating polypeptide hormone obtained from the urine of pregnant women.

Its potency is not less than USP Chorionic Gonadotropin Units in each mg, and not less than percent and not more than percent of the potency stated on the label. However, although the assay method is specific, its precision depends on the number of animals used and is relatively low even with large numbers.

A source of homogeneous FSH that could be standardized with respect to mass and bioactivity (Keene et Cited by: In the fusion screen assay, only a 1/10 serum concentration is tested in duplicate. If strong inhibition ( syncytia) is noted for a particular serum in the screen, it is considered positive and retested at multiple dilutions to determine its titer.

HUMAN CHORIONIC GONADOTROPIN (hCG) ENZYME IMMUNOASSAY TEST KIT Catalog Number: β - hCG % α - HCG % hLH % hFSH % hTSH % Precision a.

Intra Assay variation Within-run precision was determined by replicate determination of three different control in one assay. The within assay variability is shown below: Size: KB. Immunology & Assay Development. Inimmune employs technical experts in both in vitro and in vivo analysis of immune modulation.

Our staff have extensive technical expertise in all facets of immunological analysis including but not limited to: intracellular cytokine staining and FACS analysis, primary human cell isolation, culture and analysis.

Click on the article title to read by: 6.Human chorionic gonadotrophin (serum, plasma; urine) 1 Name and description of analyte Name of analyte Human chorionic gonadotrophin (hCG; referred to by this abbreviation throughout article) Alternative names Chorionic gonadotrophin, choriogonadotrophin.

The terms beta-HCG and β-HCG are no longer recommended. Description of analyteFile Size: KB.immunologic pregnancy test: a general term for tests for detection of increased human chorionic gonadotropin in plasma or urine by immunologic techniques including latex particle agglutination, hemagglutination inhibition, radioimmunoassay, radioreceptor assays, and enzyme immunoassays.